02815nas a2200181   4500000000100000008004100001100001400042700002100056700001800077700002000095700001600115700001700131245009700148300001400245490000800259520235200267022001402619       2017                            d1 aBerend N.1 aFarrow Catherine1 aSalome Cheryl1 aHarris Benjamin1 aBailey Dale1 aKing Gregory00aPeripheral ventilation heterogeneity determines the extent of bronchoconstriction in asthma.  a1188-11940 v1233 a<p>In asthma, bronchoconstriction causes topographically heterogeneous airway narrowing, as measured by three-dimensional ventilation imaging. Computation modeling suggests that peripheral airway dysfunction is a potential determinant of acute airway narrowing measured by imaging. We hypothesized that the development of low-ventilation regions measured topographically by three-dimensional imaging after bronchoconstriction is predicted by peripheral airway function. Fourteen asthmatic subjects underwent ventilation single-photon-emission computed tomography/computed tomography scan imaging before and after methacholine challenge. One-liter breaths of Technegas were inhaled from functional residual capacity in upright posture before supine scanning. The lung regions with the lowest ventilation (Vent) were calculated using a thresholding method and expressed as a percentage of total ventilation (Vent). Multiple-breath nitrogen washout was used to measure diffusion-dependent and convection-dependent ventilation heterogeneity (Sacin and Scond, respectively) and lung clearance index (LCI), before and after challenge. Forced expiratory volume in 1 s (FEV) was 87.6 ± 15.8% predicted, and seven subjects had airway hyperresponsiveness. Ventat baseline was unrelated to spirometry or multiple-breath nitrogen washout indices. Methacholine challenge decreased FEVby 23 ± 5% of baseline while Ventincreased from 21.5 ± 2.3%Ventto 26.3 ± 6.7%Vent(= 0.03). The change in Ventwas predicted by baseline Sacin ( = 0.60,= 0.03) and by LCI ( = 0.70,= 0.006) but not by Scond ( = 0.30,= 0.30). The development of low-ventilation lung units in three-dimensional ventilation imaging is predicted by ventilation heterogeneity in diffusion-dependent airways. This relationship suggests that acinar ventilation heterogeneity in asthma may be of mechanistic importance in terms of bronchoconstriction and airway narrowing.Using ventilation SPECT/CT imaging in asthmatics, we show induced bronchoconstriction leads to the development of areas of low ventilation. Furthermore, the relative volume of the low-ventilation regions was predicted by ventilation heterogeneity in diffusion-dependent acinar airways. This suggests that the pattern of regional airway narrowing in asthma is determined by acinar airway function.</p>  a1522-1601